{"id":1070,"date":"2021-08-25T13:33:28","date_gmt":"2021-08-25T04:33:28","guid":{"rendered":"https:\/\/dep.sit.ac.jp\/lsgc\/?p=1070"},"modified":"2024-08-30T10:32:03","modified_gmt":"2024-08-30T01:32:03","slug":"column10","status":"publish","type":"post","link":"https:\/\/dep.sit.ac.jp\/lsgc\/column10\/","title":{"rendered":"\u30b2\u30ce\u30e0\u7de8\u96c6"},"content":{"rendered":"\r\n

2020\u5e74\u5ea6\u306e\u30ce\u30fc\u30d9\u30eb\u5316\u5b66\u8cde\u306f\uff0c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u7814\u7a76\u306b\u3088\u3063\u3066\uff0c\u30c9\u30a4\u30c4\u306e\u30de\u30c3\u30af\u30b9\u30d7\u30e9\u30f3\u30af\u611f\u67d3\u751f\u7269\u5b66\u7814\u7a76\u6240\u306e\uff0c\u30a8\u30de\u30cb\u30e5\u30a8\u30eb\u30fb\u30b7\u30e3\u30f3\u30d1\u30f3\u30c6\u30a3\u30a8\u535a\u58eb\u3068\u30a2\u30e1\u30ea\u30ab\u306e\u30ab\u30ea\u30d5\u30a9\u30eb\u30cb\u30a2\u5927\u5b66\u30d0\u30fc\u30af\u30ec\u30fc\u6821\u306e\u30b8\u30a7\u30cb\u30d5\u30a1\u30fc\u30fb\u30c0\u30a6\u30c9\u30ca\u535a\u58eb\u306e2\u4eba\u304c\u53d7\u8cde\u3057\u307e\u3057\u305f\u3002<\/p>\r\n\r\n\r\n\r\n

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\u3053\u308c\u307e\u3067\uff0c\u30b2\u30ce\u30e0DNA\u306e\u5207\u65ad\u306b\u306f\uff0c\u5236\u9650\u9175\u7d20\u304c\u4f7f\u308f\u308c\u3066\u304d\u307e\u3057\u305f\u304c\uff0c\u9577\u3044DNA\u306e\u4e00\u30ab\u6240\u3060\u3051\u3092\u5207\u65ad\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u305d\u3053\u3067\uff0c\u7d30\u83cc\u304c\u3082\u3063\u3066\u3044\u308b\u30d5\u30a1\u30fc\u30b8\u611f\u67d3\u306e\u9632\u5fa1\u6a5f\u69cb\u3067\u3042\u308b\uff0cCRISPR\/Cas9\u3068\u3044\u3046\u30b7\u30b9\u30c6\u30e0\u3092\u5229\u7528\u3057\u3066\uff0c\u30b2\u30ce\u30e0DNA\u306e\u4efb\u610f\u306e\u5834\u6240\u3092\u4e00\u30ab\u6240\u3060\u3051\u5207\u65ad\u3067\u304d\u308b\u3088\u3046\u306b\u3057\u305f\u306e\u304c\uff0c\u4eca\u56de\u30ce\u30fc\u30d9\u30eb\u8cde\u3092\u53d7\u8cde\u3057\u305f\uff0c\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u3067\u3059\u3002CRISPR\/Cas9\u3068\u306f\uff0cClustered Regularly Interspaced Short Palindromic Repeats\/CRISPR-Associated Protein 9\u3092\u7565\u3057\u305f\u3082\u306e\u3067\u3059\u3002<\/p>\r\n\r\n\r\n\r\n

\u307e\u305a\uff0c\u306f\u3058\u3081\u306bCRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306b\u3064\u3044\u3066\uff0c\u7c21\u5358\u306b\u8aac\u660e\u3057\u307e\u3059\u3002\uff08\u56f31\u53c2\u7167\uff09<\/p>\r\n\r\n\r\n\r\n

\"\"<\/figure>\r\n\r\n\r\n\r\n

\u7d30\u83cc\u306b\u30d5\u30a1\u30fc\u30b8\u304c\u611f\u67d3\u3057\uff0c\u30d5\u30a1\u30fc\u30b8DNA\u304c\u7d30\u83cc\u5185\u306b\u4fb5\u5165\u3059\u308b\u3068\uff0c\u5207\u65ad\u3055\u308c\u3066\uff0c\u30b9\u30da\u30fc\u30b5\u30fc\u914d\u5217\u3068\u3057\u3066\u7d30\u83cc\u306e\u30b2\u30ce\u30e0\u4e2d\u306eCRISPR\u5ea7\u4f4d\u306b\u53d6\u308a\u8fbc\u307e\u308c\uff0c\u4fdd\u5b58\u3055\u308c\u305f\u30d1\u30ea\u30f3\u30c9\u30ed\u30fc\u30e0\u914d\u5217(\u56de\u6587\u914d\u5217)\u3092\u3082\u3064\u53cd\u5fa9\u914d\u5217\u3067\u5206\u96e2\u3055\u308c\u307e\u3059\u3002\u305d\u3053\u306b\uff0c\u65b0\u305f\u306b\u30d5\u30a1\u30fc\u30b8\u304c\u611f\u67d3\u3057\uff0c\u540c\u4e00\u306e\u30d5\u30a1\u30fc\u30b8DNA\u304c\u7d30\u83cc\u5185\u306b\u4fb5\u5165\u3059\u308b\u3068\uff0c\u30b9\u30da\u30fc\u30b5\u30fc\u914d\u5217\u304ccrRNA\u3068\u547c\u3070\u308c\u308b\u77ed\u9396RNA\u3068\u3057\u3066\u767a\u73fe\u3057\u307e\u3059\u3002\u3053\u306ecrRNA\u306f\u7d30\u83cc\u30b2\u30ce\u30e0\u306e\u5225\u306e\u5834\u6240\u304b\u3089\u8ee2\u5199\u3055\u308c\u305ftracrRNA\u3068\u547c\u3070\u308c\u308bRNA\u3068\u53cd\u5fa9\u914d\u5217\u90e8\u5206\u3067\u7d50\u5408\u3057\uff0c\u3055\u3089\u306bcrRNA\u3068\u76f8\u88dc\u7684\u306a\u914d\u5217\u3092\u3082\u3064\u65b0\u305f\u306b\u4fb5\u5165\u3057\u305f\u30d5\u30a1\u30fc\u30b8DNA\u3068\u7d50\u5408\u3059\u308b\u3068\uff0ctracrRNA\u306b\u7d50\u5408\u3057\u305fCas9\u3068\u547c\u3070\u308c\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u3063\u3066\uff0c\u30d5\u30a1\u30fc\u30b8DNA\u3092\u4e8c\u672c\u9396\u5207\u65ad\u3057\uff0c\u30d5\u30a1\u30fc\u30b8\u611f\u67d3\u3092\u9632\u5fa1\u3057\u307e\u3059\u3002<\/p>\r\n\r\n\r\n\r\n

\u3000<\/strong>\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u306f\uff0cCRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306ecrRNA\u306e\u4ee3\u308f\u308a\u306b\uff0c\u5207\u65ad\u3057\u305f\u3044\u30b2\u30ce\u30e0\u4e0a\u306e\u5869\u57fa\u914d\u5217\u3068\u76f8\u88dc\u7684\u306a\u914d\u5217\u3092\u3082\u3064RNA\u3068tracrRNA\u3068\u9023\u7d50\u3057\u305fgRNA\uff08\u30ac\u30a4\u30c9RNA\uff09\u3092\u4eba\u5de5\u7684\u306b\u5408\u6210\u3057\uff0cCas9\u3068\u3068\u3082\u306b\uff0c\u76ee\u7684\u306e\u30b2\u30ce\u30e0\u3092\u3082\u3064\u7d30\u80de\u5185\u306b\u5c0e\u5165\u3059\u308b\u3053\u3068\u306b\u3088\u308a\uff0c\u30b2\u30ce\u30e0\u4e0a\u306e\u4efb\u610f\u306e\u5834\u6240\u3092\u4e8c\u672c\u9396\u5207\u65ad\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3000\u305d\u306e\u5f8c\uff0c\u5207\u65ad\u3055\u308c\u305f\u30b2\u30ce\u30e0DNA\u304c\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\uff08NHEJ\uff09\u306b\u3088\u308a\u4fee\u5fa9\u3059\u308b\u3068\uff0c\u4e00\u90e8\u306eDNA\u304c\u6b20\u5931\u3059\u308b\u3053\u3068\u306b\u3088\u308a\uff0c\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u304c\u7834\u58ca\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u3092\uff0c\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3068\u3044\u3044\u307e\u3059\u3002\u307e\u305f\uff0c\u4e8c\u672c\u9396\u5207\u65ad\u3055\u308c\u305f\u5834\u6240\u306b\u76f8\u540c\u7d44\u63db\u3048\u4fee\u5fa9\uff08HDR\uff09\u306b\u3088\u308a\uff0c\u65b0\u305f\u306b\u5225\u306e\u907a\u4f1d\u5b50\u3092\u633f\u5165\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u3092\uff0c\u907a\u4f1d\u5b50\u30ce\u30c3\u30af\u30a4\u30f3\u3068\u3044\u3044\u307e\u3059\u3002\uff08\u56f32\u53c2\u7167\uff09<\/p>\r\n\r\n\r\n\r\n

\"\"<\/figure>\r\n\r\n\r\n\r\n

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1\u3064\u76ee\u306f\uff0c\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u6280\u8853\u3067\u306f\uff0c\u5916\u6765\u306e\u907a\u4f1d\u5b50\u3092\u5916\u304b\u3089\u5c0e\u5165\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u3059\u304c\uff0c\u3059\u3067\u306b\u3042\u308b\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u4f8b\u3048\u3070\uff0c\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u6280\u8853\u306e\u4e00\u3064\u3067\u3042\u308b\u907a\u4f1d\u5b50\u6cbb\u7642\u3067\u306f\uff0c\u6a5f\u80fd\u3057\u306a\u3044\u907a\u4f1d\u5b50\u306e\u4ee3\u308f\u308a\u306b\u6b63\u5e38\u306a\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u3059\u304c\uff0c\u75c5\u6c17\u306e\u539f\u56e0\u3068\u306a\u308b\u7570\u5e38\u306a\u907a\u4f1d\u5b50\u81ea\u4f53\u3092\u7834\u58ca\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u305d\u308c\u306b\u5bfe\u3057\u3066\uff0c\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u306f\uff0c\u7570\u5e38\u306a\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3057\u305f\u4e0a\u3067\uff0c\u6b63\u5e38\u306a\u907a\u4f1d\u5b50\u3092\u5c0e\u5165\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\r\n\r\n\r\n\r\n

2\u3064\u76ee\u306f\uff0c\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u4f5c\u7269\u3092\u683d\u57f9\u3059\u308b\u5834\u5408\u306b\u306f\uff0c\u30ab\u30eb\u30bf\u30d8\u30ca\u6cd5\u306b\u3088\u308a\u69d8\u3005\u306a\u5236\u9650\u304c\u3042\u308a\uff0c\u307e\u305f\u907a\u4f1d\u5b50\u7d44\u63db\u3048\u4f5c\u7269\u81ea\u4f53\u304c\u4e16\u306e\u4e2d\u306b\u53d7\u3051\u5165\u308c\u3089\u308c\u3066\u3044\u306a\u3044\u73fe\u72b6\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u308c\u306b\u5bfe\u3057\uff0c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3092\u4f7f\u3048\u3070\uff0c\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u3092\u7834\u58ca\u3059\u308b\u3060\u3051\u306a\u306e\u3067\uff0c\u30ab\u30eb\u30bf\u30d8\u30ca\u6cd5\u306b\u62b5\u89e6\u3059\u308b\u3053\u3068\u306a\u304f\uff0c\u30b2\u30ce\u30e0\u7de8\u96c6\u4f5c\u7269\u3092\u683d\u57f9\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\r\n\r\n\r\n\r\n

\u3000\u3053\u306e\u3088\u3046\u306b\uff0c\u30b2\u30ce\u30e0\u7de8\u96c6\u306f\uff0c\u907a\u4f1d\u5b50\u3092\u6539\u5909\u3059\u308b\u65b0\u305f\u306a\u30c4\u30fc\u30eb\u3068\u3057\u3066\uff0c\u6ce8\u76ee\u3092\u96c6\u3081\u3066\u3044\u307e\u3059\u3002

\u6587\u8cac\uff1a\u77f3\u5ddd \u6b63\u82f1\uff08\u6559\u6388\uff09<\/a>\u3000https:\/\/www.sit.ac.jp\/laboguide\/kougaku\/seimeikankyou\/#ishikawa<\/a><\/span><\/p>\r\n","protected":false},"excerpt":{"rendered":"2020\u5e74\u5ea6\u306e\u30ce\u30fc\u30d9\u30eb\u5316\u5b66\u8cde\u306f\uff0c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u7814\u7a76\u306b\u3088\u3063\u3066\uff0c\u30c9\u30a4\u30c4\u306e\u30de\u30c3\u30af\u30b9\u30d7\u30e9\u30f3\u30af\u611f\u67d3\u751f\u7269\u5b66\u7814\u7a76\u6240\u306e\uff0c\u30a8\u30de\u30cb\u30e5\u30a8\u30eb\u30fb\u30b7\u30e3\u30f3\u30d1\u30f3\u30c6\u30a3\u30a8\u535a\u58eb\u3068\u30a2\u30e1\u30ea\u30ab\u306e\u30ab\u30ea\u30d5\u30a9\u30eb\u30cb\u30a2\u5927\u5b66\u30d0\u30fc\u30af\u30ec\u30fc\u6821\u306e\u30b8\u30a7\u30cb\u30d5\u30a1\u30fc\u30fb\u30c0\u30a6\u30c9\u30ca\u535a\u58eb\u306e2\u4eba\u304c\u53d7\u8cde\u3057 […]","protected":false},"author":4,"featured_media":1090,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-1070","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-column"],"_links":{"self":[{"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/posts\/1070","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/comments?post=1070"}],"version-history":[{"count":11,"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/posts\/1070\/revisions"}],"predecessor-version":[{"id":3415,"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/posts\/1070\/revisions\/3415"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/media\/1090"}],"wp:attachment":[{"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/media?parent=1070"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/categories?post=1070"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dep.sit.ac.jp\/lsgc\/wp-json\/wp\/v2\/tags?post=1070"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}